Freshwater macroinvertebrates are commonly used as indicators of stream condition. Various sampling methods have been developed to ensure a consistent approach to sampling is undertaken. Underpinning these methods is the need to conduct seasonal surveys, which traditionally occur in spring and autumn. The reason for a seasonal approach is to capture specimens at an ideal life stage when identification is easily achieved. This is certainly the case with many invertebrate taxa; especially those that inhabit alpine and sub-alpine streams with short aquatic life-stages where growth and reproductive stages are strongly influenced by the onset of cold winters and short summers. However in the more temperate Sydney basin, where daytime temperatures seldom drop below 10 degrees Celsius, seasonal differences in the invertebrate community may not be as pronounced. In this study we investigate if seasonality exists in invertebrate communities of the Sydney basin. We use family level macroinvertebrate data collected from streams across the Sydney Basin to determine if differences in community assemblages vary between spring and autumn.  The implications of the findings of this study may influence invertebrate monitoring programs across the region and lead to the refinement of sampling programs and allocation of appropriate resources.